DUBAI, United Arab Emirates (AP) — A U.S. warship based in Norfolk fired warning shots when vessels of Iran’s paramilitary Revolutionary Guard came too close to a patrol in the Persian Gulf, the U.S. Navy said Wednesday.

The Navy released black-and-white footage of the encounter Monday night in international waters of the northern reaches of the Persian Gulf. In it, lights can be seen in the distance and what appears to be a single gunshot can be heard, with a tracer round racing across the top of the water.

Iran did not immediately acknowledge the incident.

The Navy said the USS Firebolt, which is based at Joint Expeditionary Base Little Creek, fired the warning shots after three fast-attack Guard vessels came within 62 meters of it and the U.S. Coast Guard patrol boat USCGC Baranoff.

“The U.S. crews issued multiple warnings via bridge-to-bridge radio and loud-hailer devices, but the (Guard) vessels continued their close range maneuvers,” said Cmdr. Rebecca Rebarich, a spokeswoman for the Mideast-based 5th Fleet. “The crew of Firebolt then fired warning shots, and the (Guard) vessels moved away to a safe distance from the U.S. vessels.”

She called on the Guard to “operate with due regard for the safety of all vessels as required by international law.”

“U.S. naval forces continue to remain vigilant and are trained to act in a professional manner, while our commanding officers retain the inherent right to act in self-defense,” she said.

The incident Monday marked the second time the Navy accused the Guard of operating in an “unsafe and unprofessional” manner this month alone after tense encounters between the forces had dropped in recent years.

Footage released Tuesday by the Navy showed a ship commanded by the Guard cut in front of the USCGC Monomoy, causing the Coast Guard vessel to come to an abrupt stop with its engine smoking on April 2.

The Guard also did the same with another Coast Guard vessel, the USCGC Wrangell, Rebarich said. Such close passes risk collisions.

The interaction marked the first “unsafe and unprofessional” incident involving the Iranians since April 15, 2020, Rebarich said. However, Iran had largely stopped such incidents in 2018 and nearly in the entirety of 2019, she said.

In 2017, the Navy recorded 14 instances of what it describes as “unsafe and or unprofessional” interactions with Iranians forces. It recorded 35 in 2016, and 23 in 2015.

The incidents at sea almost always involve the Revolutionary Guard, which reports only to Supreme Leader Ayatollah Ali Khamenei. Typically, they involve Iranian speedboats armed with deck-mounted machine guns and rocket launchers test-firing weapons or shadowing American aircraft carriers passing through the Strait of Hormuz, the narrow mouth of the Persian Gulf through which 20% of all oil passes.

Some analysts believe the incidents are meant in part to squeeze President Hassan Rouhani’s administration after the 2015 nuclear deal. They include a 2016 incident in which Iranian forces captured and held overnight 10 U.S. sailors who strayed into the Islamic Republic’s territorial waters.

The incident comes as Iran negotiates with world powers in Vienna over Tehran and Washington returning to the 2015 nuclear deal. It also follows a series of incidents across the Mideast attributed to a shadow war between Iran and Israel, which includes attacks on regional shipping and sabotage at Iran’s Natanz nuclear facility.